Relationship between Bleomycin hydrolase and Apolipoprptein E genes in Alzheimer patients in Northwest of Iran

Background and Aims: Alzheimer's disease (AD) is the most common and devastating neurodegenerative disease of the elderly. Several studies indicated a relationship between different genes and AD. Bleomycin hydrolase (BLMH) and the Apolipoprotein E (ApoE) gene allele epsilon4 (E4) known as genetic risk factors for late-onset AD in sporadic cases. We have analyzed the association between the Apo E and BLMH polymorphisms in AD and control group in Northwest of Iran. Methods: EDTA blood from 62 AD and 65 controls were collected and DNA was extracted. The genes were amplified with SSCP-PCR for BLMH and RFLP-PCR for ApoE and allelic frequencies were performed. Then the results compared between AD cases and control group by chisquared test. Results: Apo E4 and BLMH alleles frequencies are %8.33 Control, %18.33 AD and %20 Control, %36 AD respectively. Conclusion: According with our result, no association was observed between carrying the G allele of BLMH gene and AD in epsilon4 negative groups but carrying the epsilon4 allele is a dose-dependent risk factor for the AD and decrease the age of symptom onset (p < 0.05). Finally when considering the ApoE and BLMH polymorphisms alone, ApoE4 status is the best predictor of AD. [Seyed Mahmoud Tabatabaei. Relationship between Bleomycin hydrolase and Apolipoprptein E genes in Alzheimer patients in Northwest of Iran. Life Sci J 2012;9(4):4338-4340]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 650